Mouse monoclonal antibody
Cat.No. HYB 130-14
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Tetranectin (TN) is a serum and tissue protein, a C-type lectin, which binds to Ca++. It is a homotrimer of monomers each with a mass of 20 kDa, plasma or serum concentrations of TN are found to be approximately 10 mg/l (1,2,4). In vitro, TN can bind to kringle 4 of plasminogen and enhance the activation of plaminogen to plasmin, catalyzed by tissue plasminogen activator in the presence of poly-D-lysine (3). TN is best known as a prognostic marker in ovarian cancer.
Tetranectin purified from human citrate plasma (3) and coupled to PPD. Boosted before fusion with recombinant tetranectin produced in E. coli and adsorbed onto aluminum hydroxide gel.
HYB 130-14 is specific for amino acids 17-181 of human tetranectin monomer
Epitopespecificity is shared or identical with HYB 130-10, is shared with HYB 130-11 and HYB 130-13 and is partly shared or different from HYB 130-12, as determined by inhibition ELISA (4).
HYB 130-14 reacts strongly with tetranectin. A strong reaction is seen in sandwich ELISA in combination with a polyclonal antibody against tetranectin (eg. DAKO A0371). HYB 130-14 works especially well as capture antibody in combination with HYB 130-11 in sandwich ELISA (4).
In western blotting HYB 130-14 reacts strongly with TN monomer and slightly with TN trimer. In fresh frozen tissues of ovarian cancer, HYB 130-14 shows no staining for TN and no staining of paraffin-embeded, microwave treated tissues.
1. Holtet TL, Graversen JH, Clemmensen I, Thogersen HC, Etzerodt M (1997) Tetranectin, a trimeric plasminogen binding C-type lectin. Protein Sci 6:1-5.
2. Nielsen BB, Kastrup JS, Rasmussen H, Holtet TL, Graversen JH, Etzerodt M, Thogersen HC, Larsen IK (1997) Crystal structure of tetranectin, a trimeric plasminogen-binding protein with an alpha-helical coiled coil. FEBS Lett. 412:388-96.
3. Clemmensen I, Petersen LC, Kluft C (1986) Purification and characterization of a novel, oligomeric, plasminogen kringle 4 binding protein from human plasma: Tetranectin. Eur J Biochem 156:327-33.
4. Hogdall CK, Christiansen M, Christensen L, Yazova AK, Koch C, Clemmensen I, Norgaard-Pedersen B (1997) Monoclonal antibodies against human tetranectin, epitope characterization and use in immunohistochemistry. Clin Chim Acta 258:159-177.
5. Hogdall CK (1998) Human tetranectin: methodological and clinical studies. APMIS Suppl 86:1-31.
6. Thougaard AV, Hogdall CK, Kjaer SK, Blaakaer J, Jaliashvili I, Christiansen M (1998) Determination of serum tetranectin: technical and clinical evaluation of three sandwich immunoassays. Clin Chim Acta 276:19-34.
7. Thougaard AV, Jaliashvili I, Christiansen M (2001) Tetranectin-like protein in vertebrate serum: a comparative immunochemical analysis. Comp Biochem Physiol B Biochem Mol Biol 128:625-634.
8. Westergaard UB, Andersen MH, Heegaard CW, Fedosov SN, Petersen TE (2003) Tetranectin binds hepatocyte growth factor and tissue-type plasminogen activator. Eur J Biochem 270:1850-1854.