Anti-Fibronectin (human)
Mouse monoclonal antibody
Cat.No. HYB 051-05
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities.
Fibronectin isolated from human plasma adsorbed onto aluminum hydroxide gel
HYB 051-05 has specificity for human fibronectin
Not determined
HYB 051-05 reacts strongly with fibronectin. A strong reaction is seen in ELISA with fibronectin directly coated onto the microtiter plate.
In Western blot after SDS-PAGE, HYB 051-05 reacts with fibronectin in reduced as well as in nonreduced form.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Yes | |
| Immunohistochemistry | Not determined |
1. Underwood PA, Dalton BA, Steele JG, Bennett FA, Strike P (1992) Anti-fibronectin antibodies that modify heparin binding and cell adhesion: evidence for a new cell binding site in the heparin binding region. J Cell Sci 102:833-845.
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HYB 051-05.pdf (34.79 kB) Product specification
Monoclonal antibodies for extracellular matrix proteins - Flyer.pdf (645.54 kB)
Monoclonal antibodies for extracellular matrix proteins flyer
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April 23 2008
