Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Type I collagen is present in connective tissue and bone where it makes up about 90% of the organic matrix. Defects in one or both alleles of the type I collagen gene causes "brittle bone disease" (osteogenesis imperfecta). Patients suffering from such defects have low levels of fully functional type I collagen resulting in fragile bones.

Pepsin-solubilized, salt-fractioned type I collagen from human placenta (1)

CSI 008-01 is highly specific for native type I collagen and shows no evidence of cross-reactivity with type II, III, V and VI collagens in inhibition ELISA and immunoblotting, and no evidence of cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). CSI 008-01 reacts with human, calf, sheep, pig, dog and goat type I collagens as demonstrated by ELISA and immunoblotting, but does not cross-react with mouse, guinea pig, cat, rabbit, squirrel, horse, kangaroo or chicken type I collagen. CSI 008-01 has a Ka of approximately 2.5 x 106 M-1 for human type I collagen.

Epitope is located near the C-terminal of the molecule

CSI 008-01 can be used to detect collagens by ELISA. In immunoblotting CSI 008-01 detects human type I collagen only in its native triple helix form. CSI 008-01 can be used for immunohistochemistry on frozen unfixed sections of human (1,4), and bovine (2,3) skin. If fixation of tissue is required, acetone or ethanol is recommended.