Anti Type V Collagen
Mouse monoclonal antibody
Cat.No. CSI 006-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Type V collagen is a minor component of the connective tissue, although it is present in many different types of connective tissue. Patients with defects in the type V collagen (Ehlers-Danlos syndrome) have weakend connective tissue characterized by hyperstrechable joints and fragile, easily bruisable skin.
Acid-digested pepsin soluble dog type V collagen
CSI 006-01 is highly specific for type V collagen. It has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
CSI 006-01 shows reactivity with human, dog, sheep, pig, kangaroo (1), rabbit (5) and calf (2) type V collagens by ELISA and immunoblotting. CSI 006-01 does not show reactivity with mouse, rat, guinea pig and chicken (1) type V collagens by ELISA and immunoblotting.
Not determined
CSI 006-01 can be used for detection of collagens by ELISA. CSI 006-01 has been used succesfully for immunohistology on paraffin embedded (5) and frozen unfixed sections of human (4), bovine (2) and dog (1) skin, on rabbit (5) and foetal bovine cornea (1), and of new dog tissue associated with a biomaterial implant (3). If fixation of tissue is required, acetone or ethanol is recommended. CSI 006-01 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:50 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Yes | 1, 2, 3, 4, 5 |
1. Werkmeister JA, Ramshaw JAM (1991) Monoclonal antibodies to type V collagen as markers for new tissue deposition associated with biomaterial implants. J Histochem Cytochem 39:1215-1220.
2. Werkmeister JA, Ramshaw JAM (1988) The use of immunohistology in studies on connective tissue organisation in hides and skins. Das Leder 39:145-151.
3. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.
4. Werkmeister JA, Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of human skin. Acta Derm Venereol 69:399-402.
5. Pollock GA, McKelvie PA, McCarty DJ, White JF, Mallari PL, Taylor HR. (2003) In vivo effects of fluoroquinolones on rabbit corneas. Clin Experiment Ophthalmol 31:517-21.
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CSI 006-01.pdf (12.61 kB) Product specification
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April 23 2008
