Anti-Pregnancy associated plasma protein A (human, PAPP-A)

Cat.No. BTE 004-09

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Cat. No.
BTE 004-09
Product name
Anti-Pregnancy associated plasma protein A (human, PAPP-A)
Description

Mouse monoclonal antibody

Specificity

BTE 004-09 is specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A.

Immunogen

Purified human PAPP-A

Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG2b/k

Gene ID
5069
Clone number
9
Epitope

The epitop of BTE 004-09 is different from ABS 006-01 and ABS 006-24. The epitope is located on the PAPP-A subunit of the heterotetramer.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterotetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (trisomy 21).

Application 1

ELISA
In sandwich ELISA the optimal combination is BTE 004-09 used as coating antibody and biotinylated ABS 006-24 used as detection antibody. BTE 004-09 can also be used as detection antibody in sandwich ELISA  in combination with a polyclonal antibody towards PAPP-A in coat. 

Application 2

WB
BTE 004-09 detect both reduced and unreduced PAPP-A in Western Blotting, though reaction to unreduced is less than that of ABS 006-01 and ABS 006-24.

Application 3

IHC
BTE 004-09 can be used in IHC.
 

Staining is restricted to the syncytiotrophoblast of the chorionic villi in frozen human placenta tissue, stained with BTE 004-09.

Staining is restricted to the syncytiotrophoblast of the chorionic villi in paraffin embedded human placenta tissue, stained with BTE 004-09.