Anti-Antithrombin III (human, native)

Cat.No. HYB 230-01B

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Cat. No.
HYB 230-01B
Product name
Anti-Antithrombin III (human, native)
Description

Mouse monoclonal antibody, Biotinylated

Specificity

HYB 230-01 is specific for human Antithrombin III (AT III) native form

Immunogen

Antithrombin III isolated from human plasma

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Clone number
1 B7
Epitope

Epitope specificity differs from that of HYB 230-04, HYB 228-10 and HYB 228-12.

Content

Available in 100 µL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Antithrombin III (AT III) is a single chain glycoprotein of molecular mass 58 kDa with four glycosylation sites and three intra-molecular disulfide bonds. AT III is normally present in human plasma at a concentration of approx. 12.5 mg/dl. It is the major inhibitor of thrombin. Inactivation of thrombin by AT-III occurs by formation of a covalent bond resulting in an inactive 1:1 stoichiometric complex between the two components. 

Application 1

ELISA

HYB 230-01 reacts strongly with native AT III. Strong reaction is seen in ELISA with native AT III coated directly onto the microtiter well. In a sandwich ELISA setup, HYB 230-01B acts as a detection antibody in conbination with ABS 014-22 as capture antibody to measure Thrombin-Antithrombin (TAT) complexes. 
 
The calibration curve of a sandwich assay for TAT (Thrombin-antithrombin) using ABS 014-22 as capture antibody and HYB 230-01B as the biotinylated detection antibody.