APC-PCI Complex specific antibody

Cat.No. JST 001-38

Buy online
Cat. No.
JST 001-38
Product name
APC-PCI Complex specific antibody
Description

Mouse monoclonal antibody

Specificity

JST 001-38 is specific for APC:PCI complex and cleaved PCI but has little reactivity towards native PCI. The dissociation constants (as determined by standard surface plasmon resonance technique) are: Kd= 4x10e-10 M for PCI in complex with APC and 2x10e-10 M for cleaved PCI. The value of Kd for native PCI is too low to measure, i.e. > 10e-5 M.

Immunogen

Mixture of PCI in complex with PSA (approx. 80%) and PCI cleaved from such a complex (approx. 20%).

Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
5104
Clone number
M36
Epitope

The epitope is loctated in the calcium-binding N-terminal EGF domain of protein C.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Activated Protein C (APC), a serine proteinase is inhibited by Protein C inhibitor (PCI). PCI belongs to a group of inhibitors sometimes referred to as serpines (serine proteinase inhibitors), and form 1:1 complexes with APC. PCI plasma concentration is approximately 4ug/m1 .The complex formation between APC and PCI proceeds at a slow rate, which is manifested by a long half-life for APC in plasma, wherein t1/2 is about 20 min.However, the rate of complex formation between APC and PCI is increased by heparin.Upon complex formation with APC, the serpin is cleaved in its so-called bait region, whereby a stable intermediate acyl complex is formed. With time, the intermediate acyl complex dissociates, whereby APC is regenerated and a proteolytically modified, i. e. cleaved, inactive serine proteinase inhibitor is formed.

Application 1

ELISA
JST 001-38 shows excellent reactivity towards PCI in cleaved and complex form in ELISA. JST 001-38 is a part of the APC-PCI Matched Reagent Set, and can be used in ELISA as a capture antibody in combination with SA001RA as the detection antibody (1-5).
 

The figure illustrates the calibration curve of a sandwich assay with JST 001-38 as the capture antibody and SA001RA as the biotinylated detection antibody.

 

Application 2

WB
JST 001-38 shows low reactivity against denatured antigen in SDS-PAGE. 

1.Strandberg K, Astermark J, Björgell O, Becker C, Nilsson PE, Stenflo J (2001) Complexes between activated protein C and protein C inhibitor measured with a new method: comparison of performance with other markers of hypercoagulability in the diagnosis of deep vein thrombosis. Thromb Haemost 86:1400-08.

2.Bremme K, Hamad R, Berg E, Strandberg K, Stenflo J (2012) The APC-PCI concentration as an early marker of activation of blood coagulation: a study of women on combined oral contraceptives. Thromb Res.130:636-9.

3. Elf JL, Strandberg K, Svensson PJ (2010) The diagnostic performance of APC-PCI complex determination compared to D-dimer in the diagnosis of deep vein thrombosis. J Thromb Thrombolysis. 29:465-70.

4.Bhiladvala P, Strandberg K, Stenflo J, Holm J (2006) Early identification of acute myocardial infarction by activated protein C-protein C inhibitor complex. Thromb Res 118:213-19.

5.Heslet L, Hald R, Recke C, Bangert K, Uttenthal LO (2008) Activated protein C-protein C inhibitor (APC-PCI) complex as a prognostic marker in sepsis. Presented at the The International Sepsis Forum (ISF) 2008, Granada, Spain. 

Request

Further information