NGAL ELISA Kit (human)

Cat.No. KIT 036

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Cat. No.
KIT 036
Product name
NGAL ELISA Kit (human)
Description
An ELISA for the in vitro determination of human NGAL in urine, serum, plasma, tissue extracts or culture media.
 
Assay range
10-1000 pg/mL
Assay time
4 hours
Limit of detection
4 pg/mL
Sample dilution
1/500 (Urine from healthy subjects)
Species reactivity (positive)
None
Storage
4°C
Target
Human NGAL (Neutrophil Gelatinase-Associated Lipocalin)
Background
NGAL is a new early biomarker for diagnosing acute kidney injury (AKI)
 
Under normal conditions, NGAL levels are low in urine and plasma, but they rise sharply from basal levels in response to kidney injury to reach diagnostic levels within a very short time - as much as 24 hours or more before any significant rise in serum creatinine.
Regulatory status
For research use only. Not for use in diagnostic procedures.
Other information
Simple ELISA format - easy to perform Ready-to-use calibrators and working solutions Pre-coated ELISA strips All incubations performed at room temperature Easy storage All components are stored at 4°C Automation possible on open ELISA workstations
Hasse-Fielitz A, Haase M, Devarajan P (2014) Neutrophil gelatinase-associated lipocalin as a biomarker of acute kidney injury: a critical evaluation of current status. Ann Clin Biochem 51:335-351
Cho E, Yang HN, Jo SK, Cho WY, Kim HK (2013) The role of urinary liver-type fatty acid-binding protein in critically ill patients. J Korean Med Sci. 28:100-105
Di NM, Ficarella A, Ricci Z, Luciano R, Stoppa F, Picardo S, Picca S, Muraca M, Cogo P (2013)Impact of severe sepsis on serum and urinary biomarkers of acute kidney injury in critically ill children: an observational study. Blood Purif 35: 172-176
Kashani K et al (2013) Discovery and validation of cell cycle arrest biomarkers in human acute kidney injury. Crit Care 17:R25  
Kift RL et al (2013) A comparison of the analytical performance of five commercially available assays for neutrophil gelatinase-associated lipocalin using urine. Ann Clin Biochem. 50: 236-244