Human IgE (non-immune)

Cat.No. DIA HE1A

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Cat. No.
DIA HE1A
Product name
Human IgE (non-immune)
Description
Human Monoclonal Antibody
Specificity
This product is purified human IgE with kappa light chains produced in vitro from a monoclonal hybridoma. Original material is obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B.Must be handled as potentially infectious as all human material.The purity of the human IgE is > 90%, tested by SDS-PAGE.As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The human IgE is purified by Protein L chromatography. The remaining contaminants are mainly components from Foetal Bovine Serum.
Immunogen
Non-immune
Subclass
IgE/k
Epitope
Non-immune
Content
100 µl, 1mg/mL
Purification
Protein L purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.15 M NaCl and 0.09 % azide
Storage
For long term storage, -18 to -22ºC is recommended, but it may be stored for short term use at 2 to 8ºC. The expiry date refers to storage at -18 to -22ºC. Repeated freeze/thawing is not recommended
Application 1
ELISA
This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch variation. 
Application 2
IHC
This antibody can be used in immunochemistry
Application 3
Cell Stimulation Asssay
This antibody can be used in cellular immunology research.
Application 4
Western Blot
This antibody can be used in Western Blotting.
Specific references
Stimulation of human bronchial/tracheal smooth muscle (B/TSM) cells with IgE
1. Redhu NS, Saleh A, Shan L, Gerthoffer WT, Kung SK, Halayko AJ, Lamkhioued B, Gounni AS (2009) Proinflammatory and Th2 Cytokines Regulate the High Affinity IgE Receptor (FceRI) and IgE-Dependant Activation of Human Airway Smooth Muscle Cells. PLoS ONE 4:e6153.
IgE used in biosensor research
2. Chang C-C, Chen C-Y, Zhao X, Wu T-H, Wei S-C, Lin C-W (2014)
Label-free colorimetric aptasensor for IgE using DNA pseudoknot probe. Analyst 139:3347-3351. 24821053
Stimulation of neutrophils
3. Saffar AS, Alphonse MP, Shan L, HayGlass KT, Simons FER, Gounni AS (2007) IgE modulates neutrophil survival in asthma: Role of mitochondrial pathway. J immunol 178:2535-2541.
Investigation of Aspergillus oryzae involvement in allergic bronchopulmonary aspergillosis (ABPA)
4. Yamaki K and Yoshino S (2011) Aspergillus oryzae Lectin Induces Anaphylactoid Oedema and Mast Cell Activation Through its Interaction With Fucose of Mast Cell–Bound Non-specific IgE. Scandinavian Journal of Immunology 74:445–453.
Used as a control for IgE in skin graft on mice
5. Fairley JA, Burnett CT, Fu CL, Larson DL, Fleming MG, Giudice GJ (2007) A pathogenic role for IgE in autoimmunity: Bullous pemphigoid IgE reproduces the early phase of lesion development in human skin grafted to nu/nu mice. J Invest Dermatol 127:2605-2611.