Anti-MBL (human, horse, pig)

Cat.No. HYB 131-14

Cat. No.
HYB 131-14
Product name
Anti-MBL (human, horse, pig)
Description

Mouse monoclonal antibody

Specificity

HYB 131-14 is specific for human MBL (mannan-binding lectin) from plasma or serum, but cross-reacts strongly with MBL from horse and pig.

Immunogen

MBL purified from human donor plasma 

Application
Species reactivity (positive)
Human, horse, pig
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
4153
Clone number
15C5
Epitope

Not determined 

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Mannan-binding lectin (MBL), also called mannose-binding lectin or protein, belongs to the C-type family of collectins, showing calcium-dependent binding to certain sugars. It consists of oligomers of triple-chain subunits and its binding and complement activating activities depend on its normal oligomerization. On binding to mannan-like microbial surface carbohydrates, MBL activates the complement system by means of its own lectin pathway, depending on the MBL-associated serine proteases (MASPs). Because of the presence of different structural and promoter alleles in the population, 12% or more of the population have low concentrations (<50ng/mL) of normally oligomerized, functional MBL in plasma or serum.

Application 1

ELISA

HYB 131-14 reacts strongly with human, horse and pig serum, when captured on an mannan coat in ELISA. A weaker reaction is seen when HYB 131-14 is used as a detection antibody for human MBL coated directly onto the microtiter plate.
Application 2

WB

HYB 131-14 can be used in Western blotting.