Anti-MBL (human)

Cat.No. HYB 131-01

Cat. No.
HYB 131-01
Product name
Anti-MBL (human)

Mouse monoclonal antibody


HYB 131-01 is specific for MBL (mannan-binding lectin) from human serum or plasma.


MBL purified from human donor plasma 

Species reactivity (positive)
Species reactivity (negative)
Not determined


Gene ID
Clone number

The epitope is on the head-neck region of the MBL protein chain. Prior binding of the antibody is thought to block binding to carbohydrate. The epitope differs from that of HYB 131-10 and HYB 131-11. 


Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Protein-A purified
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
4-8ºC without exposure to light. No precautions necessary during handling.

Mannan-binding lectin (MBL), also called mannose-binding lectin or protein, belongs to the C-type family of collectins, showing calcium-dependent binding to certain sugars (1). It consists of oligomers of triple-chain subunits and its binding and complement activating activities depend on its normal oligomerization. On binding to mannan-like microbial surface carbohydrates, MBL activates the complement system by means of its own lectin pathway, dependent on the MBL-associated serine proteases (MASPs). Because of the presence of different structural and promoter alleles in the population, 12% or more of the population have low concentrations (<50ng/mL) of normally oligomerized, functional MBL in plasma or serum.

Application 1

HYB 131-01 is selective for normally oligomerized MBL when used as detection antibody in sandwich ELISA with HYB 131-01 coat (1).
HYB 131-01 can also be used in a manan assay, when coating with manan and using HYB 131-01B as the biotinylated detection antibody (2).

The calibration curve of a sandwich assay for MBL using HYB 131-01 as the capture antibody and HYB 131-01B as the biotinylated detection antibody.
Application 2

In Western blotting, HYB 131-01 reacts with human MBL in both its oligomerized state and as single protein chain of 26 kDa. A dilution guideline of 1/1000 has proved successful (2).

Application 3

HYB 131-01 is also well suited for immunohistochemistry on human tissue samples, frozen or paraffin embedded, from (3, 4). Please consult

Paraffin-embedded human liver tissue immunostained with HYB 131-01 (3)



Paraffin-embedded human cancerous liver tissue immunostained with HYB 131-01 (


1. Garred P, Madsen HO, Kurtzhals JA, Lamm LU, Thiel S, Hey AS, Svejgaard A (1992) Diallelic polymorphism may explain variations of the blood concentration of mannan-binding protein in Eskimos, but not in black Africans. Eur J Immunogenet 19:403-412.

2. Ramos GP, Nisihara R, Maestri CA, Bichalho MG, Carvalho NS, Reason IM (2013) MBL serum Concentration in women with HPV presenting CIN III lesions. Human Immunology 74:67-69

3.Garred P, Larsen F, Madsen HO,  Koch C (2003) Mannose-binding lectin deficiency - revisited. Mol Immunol 40:73-84.

4. Hornum M, Bay JT, Clausen P, Melchior Hansen J, Mathiesen ER, Feldt-Rasmussen B et al. High levels of mannose-binding lectin are associated with lower pulse wave velocity in uraemic patients. B M C Nephrology. 2014;15:162.

5. Nisihara M, Magrini F, Mocelin V, Messias-Reason IJ (2013) Deposition of the lectin pathway of complement in renal biopsies of lupus nephritis patients. Hum.Immunol. 74: 907-910