Anti-MBL (human)

Cat.No. HYB 131-01B

Cat. No.
HYB 131-01B
Product name
Anti-MBL (human)
Description

Mouse monoclonal antibody, biotinylated

Specificity

HYB 131-01 is specific for MBL (mannan-binding lectin) from human serum or plasma.

Immunogen

MBL purified from human donor plasma 

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
4153
Clone number
3B6
Epitope

The epitope is on the head-neck region of the MBL protein chain. Prior binding of the antibody is thought to block binding to carbohydrate. The epitope differs from that of HYB 131-10 and HYB 131-11. 

Content

50 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Mannan-binding lectin (MBL), also called mannose-binding lectin or protein, belongs to the C-type family of collectins, showing calcium-dependent binding to certain sugars. It consists of oligomers of triple-chain subunits and its binding and complement activating activities depend on its normal oligomerization. On binding to mannan-like microbial surface carbohydrates, MBL activates the complement system by means of its own lectin pathway, dependent on the MBL-associated serine proteases (MASPs). Because of the presence of different structural and promoter alleles in the population, 12% or more of the population have low concentrations (<50ng/mL) of normally oligomerized, functional MBL in plasma or serum.

Application 1
ELISA
HYB 131-01B is selective for normally oligomerized MBL when used as detection antibody in sandwich ELISA with HYB 131-01 coat (1, 2). HYB 131-01 can also be used in a manan assay, when coating with manan and using HYB 131-01B as the biotinylated detection antibody (3).
 
 
The calibration curve of a sandwich assay for MBL using HYB 131-01 as the capture antibody and HYB 131-01B as the biotinylated detection antibody.

1. Fiane AE, Videm V, Lingaas PS, Heggelund L, Nielsen EW, Geiran OR, Fung M, Mollnes TE (2003) Mechanism of Complement Activation and Its Role in the Inflammatory Response After Thoracoabdominal Aortic Aneurysm Repair. Circulation 108:849-856.

2. Dumestre-Perard C, Doerr E. Colombb MG, Loos M (2007) Involvement of complement pathways in patients with bacterial septicemia. Mol Immunol 44:1631-1638.

3. Ramos GP, Nisihara R, Maestri CA, Bichalho MG, Carvalho NS, Reason IM (2013) MBL serum Concentration in women with HPV presenting CIN III lesions. Human Immunology 74:67-69