Cat.No. HYB 131-11
Mouse monoclonal antibody
HYB 131-11 is specific for MBL (mannan-binding lectin) from human serum or plasma.
MBL purified from human donor plasma
The epitope is thought to be on the carbohydrate recognition domain and differs from that of HYB 131-01 and HYB 131-10.
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Mannan-binding lectin (MBL), also called mannose-binding lectin or protein, belongs to the C-type family of collectins, showing calcium-dependent binding to certain sugars. It consists of oligomers of triple-chain subunits and its binding and complement activating activities depend on its normal oligomerization. On binding to mannan-like microbial surface carbohydrates, MBL activates the complement system by means of its own lectin pathway, depending on the MBL-associated serine proteases (MASPs). Because of the presence of different structural and promoter alleles in the population, 12% or more of the population have low concentrations (<50ng/mL) of normally oligomerized, functional MBL in plasma or serum.
HYB 131-11 can be used in immunoprecipitation (3).
1. Garred P, Larsen F, Madsen HO, Koch C (2003) Mannose-binding lectin deficiency - revisited. Mol Immunol 40:73-84.
2. Skjoedt OM, Palarasah Y, Munthe-Fog L, Ma YJ, Weiss G, Skjodt K, Koch C, Garred P (2010) MBL-associated serin protease-3 circulates in high serum concentrations predominantly in complex with Ficolin-3 and regulates Ficolin-3 medicated complement activation. Immunobiology 215:921-931.
3. Skjoedt MO, Hummelshoj T, Palarasah Y, Honore C, Koch C, Skjodt K, Garred P (2010) A Novel Mannose-binding Lectin/Ficolin-associated Protein Is Highly Expressed in Heart and Skeletal Muscle Tissues and Inhibits Complement Activation. J Biol Chem 285:8234-8243.