Anti-MBL (chicken)

Cat.No. HYB 182-01

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Cat. No.
HYB 182-01
Product name
Anti-MBL (chicken)
Description

Mouse monoclonal antibody

Specificity

HYB 182-01 is specific for chicken MBL (mannan-binding lectin).

Immunogen

MBL purified from chicken plasma

Application
Species reactivity (positive)
Chicken
Species reactivity (negative)
Not determined
Subclass

IgG1/l

Gene ID
374267
Clone number
6B11
Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Mannan-binding lectin (MBL) belongs to the C-type family of collectins. It functions as an opsonin which activates complement on binding to microbial polysaccharides. Studies on chicken MBL are described in reference (1, 2).

Application 1

ELISA

In ELISA HYB 182-01 reacts strongly with chicken MBL. HYB 182-01 can be used as coating antibody (5 µg/mL) with biotinylated HYB 182-01 as detection antibody (1µg/mL) in sandwich ELISA (1).
Application 2
WB
In Western blotting a dilution guideline of 1/1000 has proved successful (2).
 
Application 3

IHC

HYB 182-01 can be used in IHC (2).

1. Juul-Madsen HR, Munch M, Handberg KJ, Sorensen P, Johnson AA, Norup LR, Jorgensen PH (2003) Serum levels of mannan-binding lectin in chickens prior to and during experimental infection with avian infectious bronchitis virus. Poult Sci 82:235-241.

2. Nielsen OL, Jorgensen PH, Hedemand J, Jensenius JC, Koch C, Laursen SB (1998) Immunohistochemical investigation of the tissue distribution of mannan-binding lectin in non-infected and virus-infected chickens. Immunology 94:122-128.

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