Anti-L-Ficolin (human)

Cat.No. ABS 005-19

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Cat. No.
ABS 005-19
Product name
Anti-L-Ficolin (human)
Description

Mouse monoclonal antibody

Specificity

ABS 005-19 reacts specifically with L-Ficolin. No cross reactivity is seen with M-Ficolin (Ficolin-1) and H-Ficolin (Ficolin-3).

Immunogen

Purified procaryot expressed recombinant human L-Ficolin and full-length L-Ficolin (Ficolin-2) protein 

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG2a/k

Gene ID
2220
Clone number
19
Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

L-Ficolin (Ficolin-2) is an innate immunity pattern recognition molecule found in human serum. L-Ficolin binds to distinct pathogen-associated molecular patterns (PAMP), such as carbohydrates (GlcNAc), lipoteichoic acid and acetylated groups thereby facilitating phagocytosis and activation of complement through the lectin route using the same serine proteases as mannose-binding lectin (MBL) named MASP ((MBL)-associated serine protease) (1).

Application 1

ELISA
ABS 005-19 is especially well suited as detecting antibody in a sandwich ELISA in combination with ABS 005-16 (1). Furthermore, in ELISA, ABS 005-19 reacts with recombinant L-Ficolin coated directly into the microtiter well.

Application 2
WB
ABS 005-19 can be used in Western blotting (1, 2, 3)
 
Application 3

IP
ABS 005-19 can be used in immunoprecipitation (4).

1.  Munthe-Fog L, Hummelshøj T, Hansen BE, Koch C, Madsen HO, Skjødt K, Garred P (2007) The impact of FCN2 polymorphisms and haplotypes on the Ficolin-2 serum levels. Scand J Immunol 65:383-392.

2. Jensen ML, Honoré C, Hummelshoj T, Hansen BE, Madsen HO, Garred P (2006) Ficolin-2 recognizes DNA and participates in the clearance of dying host cells. Mol Immunol 44:856-865.

3. Hummelshoj T, Thielens NM, Madsen HO, Arlaud GJ, Sim RB, Garred P (2006) Molecular organization of human Ficolin-2. Mol Immunol 44:401-411.

4. Skjoedt MO, Hummelshoj T, Palarasah Y, Honore C, Koch C, Skjodt K, Garred P (2010) A Novel Mannose-binding Lectin/Ficolin-associated Protein Is Highly Expressed in Heart and Skeletal Muscle Tissues and Inhibits Complement Activation. J Biol Chem 285:8234-8243.