Mouse NGAL ELISA Kit

Cat.No. KIT 042

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Cat. No.
KIT 042
Product name
Mouse NGAL ELISA Kit
Description

A quantitative assay for  in vitro the determination of mouse NGAL in urine, plasma, serum, tissue extracts or culture media.

Assay range

10-1000 pg/mL

Assay time
<4 hours
Limit of detection
<0.75 pg/mL
Sample dilution
1/1000 (Urine or EDTA plasma, from healthy mice)
Species reactivity (positive)
None
Storage
4°C
Target

Mouse NGAL (Neutrophil Gelatinase-Associated Lipocalin)

Background
Mouse NGAL - a marker for nephrotoxicity and kidney damage in pre-clinical trials
 
With the Mouse NGAL ELISA KIT, urine, plasma or serum samples can be analysed in a standard laboratory with conventional ELISA equipment. All components are stored at 4ºC and all incubations are performed at room temperature. The kit contains ready-to-use reagents and a pre-coated 96-well ELISA plate, which greatly reduces the hands-on time. The simple test protocol permits high reproducibility with a total assay time under 4 hours.
Regulatory status
RUO - Research Use Only.
Other information
Kit features Highly reproducible and easy to use Ready-to-use working solutions Ready-to-use calibrators Pre-coated 96-well ELISA plate All incubations performed at room temperature Easy storage All components are stored at 4°C Requires only standard ELISA equipment

Doi K, Katagiri D, Negishi K, Hasegawa S, Hamasaki Y, Fujita T, Matsubara T, Ishii T, Yahagi N, Sugaya T, Noiri E (2012) Mild elevation of urinary biomarkers in prerenal acute kidney injury. Kidney Int. 82:1114-1120

Srinivasan G, Aitken JD, Zhang B, Carvalho FA, Chassaing B, Shashidharamurthy R, Borregaard N, Jones DP, Gewirtz AT, Vijay-Kumar M (2012) Lipocalin 2 deficiency dysregulates iron homeostasis and exacerbates endotoxin-induced sepsis. J Immunol. 189:1911-1919

Sultan S, Pascucci M, Ahmad S, Malik IA, Bianchi A, Ramadori P, Ahmad G, Ramadori G (2012) LIPOCALIN-2 is a major acute-phase protein in a rat and mouse model of sterile abscess. Shock. 37:191-196

Morishita Y, Ohnishi A, Watanabe M, Ishibashi K, Kusano E (2011) Establishment of acute kidney injury mouse model by 0.75% adenine ingestion. Ren Fail. 33:1013-1018

Munshi R, Johnson A, Siew ED, Ikizler TA, Ware LB, Wurfel MM, Himmelfarb J, Zager RA (2011) MCP-1 gene activation marks acute kidney injury. J Am Soc Nephrol. 22:165-175