Anti-GLP-1 (Non-amidated (-Arg-Gly), C-terminal specific)

Cat.No. ABS 046-03

Cat. No.
ABS 046-03
Product name
Anti-GLP-1 (Non-amidated (-Arg-Gly), C-terminal specific)
Description

Mouse monoclonal antibody

Specificity
ABS 046-03 binds GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37).
Cross-reaction with C-terminally amidated GLP-1 is 0-16% in various circumstances.
Immunogen

Synthetic GLP-1(9-37) 

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
2641
Clone number
03
Epitope

C-terminus of GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37)

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

GLP-1(7-36) amide is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far. 

Application 1
ELISA
ABS 046-03 binds free GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37) in solution. 
ABS 046-03 works well as a capture antibody with ABS 033-10B as a biotinylated detection antibody cross-reacting about 5% with C-terminally amidated GLP-1.
ABS 046-03 can also be used as a capture antibody for detection of GLP-1 derivatives (1).
 
The calibration curve of a sandwich assay for Non-amidated GLP-1 using ABS 046-03 as the capture antibody and ABS 033-10B as the biotinylated detection antibody.
Application 2

WB

ABS 046-03 can be used in Western blotting.

1. Sang Y, Zhou T, Li H, Ran Y, Jiang D, Zheng F, Chen Y, Wang C, Zou X (2013) A new long-acting GLP-1 derivative KTP ameliorates hyperglycemia and dyslipidemia and improves pancreas and fatty liver in db/db mice. Chinese Science Bulletin 58:2447-2453.