Anti-GLP-1 (Non-amidated (-Arg-Gly), C-terminal specific)

Cat.No. ABS 046-03B

Cat. No.
ABS 046-03B
Product name
Anti-GLP-1 (Non-amidated (-Arg-Gly), C-terminal specific)
Description

Mouse monoclonal antibody, biotinylated

Specificity
ABS 046-03 binds GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37).
Cross-reaction with C-terminally amidated GLP-1 is 0-16% in various circumstances.
Immunogen

Synthetic GLP-1(9-37) 

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
2641
Clone number
03
Epitope

C-terminus of GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37)

Content

50 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

GLP-1(7-36) amide is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far. 

Application 1

ELISA
ABS 046-03B as a biotinylated detection antibody and HYB 147-12 as a capture antibody cross- reacting about 16% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized.