Anti-GLP-1 (Mid-molecule specific)

Cat.No. HYB 147-08

Cat. No.
HYB 147-08
Product name
Anti-GLP-1 (Mid-molecule specific)
Description

Mouse monoclonal antibody

Specificity

Reacts with all forms of GLP-1, including precursor and GLP-1(9-37) /GLP-1(9-36amide) metabolite. HYB 147-08 cross-reacts with Liraglutide. 

Immunogen

Synthetic GLP-1(7-36)amide coupled to carrier

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG2a/k

Gene ID
2641
Clone number
11E2
Epitope

Mid-molecular epitope of GLP-1

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Application 1

ELISA

HYB 147-08 binds to GLP-1 when coated directly onto the microtiter well, and binds GLP-1(7-36)amide in solution giving a Ka of 1.2x10E7 in inhibition ELISA. HYB 147-08 does not cross-react with coated glucagon or hGLP-2. In inhibition ELISA no binding of free glucagon in solution is detected, giving an estimated cross-reactivity of <0.2%. 
Application 2

WB

In Western blotting a dilution guideline of 1/1000 has proved successful.
Application 3

IHC

Although not tested, HYB 147-08 is likely to detect all known molecular forms of GLP-1 in immunohistochemistry (1).

1.  Voortman T, Hendriks HFJ, Witkamp RF, Wortelboer HM (2012) Effects of long- and short-chain fatty acids on the relase of gastrointestinal hormones using an ex vivo porcine intestinal tissue model. J. Agric. Food Chem. 60:9035-9042.