Anti-GLP-1(9-37) and GLP-1(9-36)amide (N-terminal specific)

Cat.No. ABS 044-49

Cat. No.
ABS 044-49
Product name
Anti-GLP-1(9-37) and GLP-1(9-36)amide (N-terminal specific)
Description

Mouse monoclonal antibody

Specificity
ABS 044-49 binds human GLP-1(9-36)amide
Based on inhibition ELISA, ABS 044-49 cross-reacts 3% with GLP-1(7-36)amide
Immunogen

Synthetic GLP-1(9-18) 

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
2641
Clone number
49
Epitope

The free N-terminus of GLP-1(9-36)amide and GLP-1(9-37)

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Glucagon-like peptide-1(9-36)amide and GLP-1(9-37) are the forms of GLP-1 that result from the rapid degradation of the active forms of the peptide (GLP-1(7-36)amide and GLP-1(7-37)) by the enzyme dipeptidyl peptidase-IV (DPP-IV, also known as CD26 or adenosine deaminase binding protein). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Application 1

ELISA

ABS 044-49 binds free GLP-1(9-36)amide in solution. In a sandwich ELISA ABS 044-49 (as capture antibody) forms a pair with  HYB 147-06B (as biotinylated detection antibody) in order to measure ”degraded GLP-1” (the GLP-1 metabolite (GLP-1(9-36)amide).
 
The calibration curve of a sandwich assay for Degraded GLP-1 using ABS 044-49 as the capture antibody and HYB 147-06B as the biotinylated detection antibody.
Application 2

WB

ABS 044-49 can be used in Western blotting.