Anti-GLP-1(9-37) and GLP-1(9-36)amide (N-terminal specific)

Cat.No. ABS 044-53

Cat. No.
ABS 044-53
Product name
Anti-GLP-1(9-37) and GLP-1(9-36)amide (N-terminal specific)
Description

Mouse monoclonal antibody

Specificity
ABS 044-53 binds human GLP-1(9-36)amide 
Based on inhibition ELISA, ABS 044-53 cross-reacts <2% with GLP-1(7-36)amide
Immunogen

Synthetic GLP-1(9-18) 

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
2641
Clone number
53
Epitope

The free N-terminus of GLP-1(9-36)amide and GLP-1(9-37)

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Glucagon-like peptide-1(9-36)amide and GLP-1(9-37) are the forms of GLP-1 that result from the rapid degradation of the active forms of the peptide (GLP-1(7-36)amide and GLP-1(7-37)) by the enzyme dipeptidyl peptidase-IV (DPP-IV, also known as CD26 or adenosine deaminase binding protein). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Application 1

ELISA

ABS 044-53 binds free GLP-1(9-36)amide in solution. ABS 044-53 has higher affinity for GLP-1(9-36)amide than ABS 044-49 as determined by inhibition.
Application 2

WB

ABS 044-53 can be used in Western blotting.