Anti-Mycobacterium tuberculosis ESAT-6

Cat.No. HYB 076-08

Cat. No.
HYB 076-08
Product name
Anti-Mycobacterium tuberculosis ESAT-6
Description

Mouse monoclonal antibody

Specificity

HYB 076-08 reacts with ESAT-6 in immunoblotting after SDS-PAGE of short-term culture filtrates of Mycobacterium tuberculosis and virulent strains of M. bovis. HYB 076-08 cross-reacts with MPB64 (26 kDa) from certain BCG substrains, with the A, B, and C components of the antigen 85 complex and with MPT51. H

Immunogen
Purified protein derivative (PPD) of tuberculin from M. tuberculosis conjugated with thimerosal N-hydroxysuccinimide ester
Application
Species reactivity (positive)
Mycobacterium tuberculosis
Species reactivity (negative)
Not determined
Subclass
IgG1/k
Clone number
11 G4
Epitope
Binds to residues 3-15 (EQQWNFAGIEAAA) of ESAT-6 (1)
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
ESAT-6 (6-kDa early secretory antigenic target) from M. tuberculosis  H37Rv (ATCC 27294) (1) is a secreted tubercular protein that forms a dominant target for cell-mediated immunity in the early phase of infection. The deduced amino acid sequence indicates a molecular mass of 10 kDa. It is present in M. tuberculosis, virulent strains of M. bovis, and in M. flavescens, M. kansasii, M. marinum,and M. szulgai, but not in avirulent M. bovis BCG substrains (3).
Application 1
ELISA
HYB 076-08 can be used in ELISA (2 ).
Application 2
WB
HYB 076-08 reacts with PPD, culture filtrate, cytosol and cell wall preparations from M. tuberculosis (2, 3, 4).
1. Harboe M, Malin AS, Dockrell HS, Wiker HG, Ulvund G, Holm A, Jørgensen MC, Andersen P (1998) B-cell epitopes and quantification of the ESAT-6 protein of Mycobacterium tuberculosis. Infect Immun 66:717-723.
2. Harboe M, Oettinger T, Wiker HG, Rosenkrands I, Andersen P (1996) Evidence for occurrence of the ESAT-6 protein in Mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG. Infect Immun 64:16-22.
3. Klausen J, Magnusson M, Andersen AB, Koch C (1994) Characterization of purified protein derivative of tuberculin by use of monoclonal antibodies: isolation of a delayed-type hypersensitivity reactive component from M. tuberculosis culture filtrate. Scand J Immunol 40:345-349.
4. Sørensen AL, Nagai S, Houen G, Andersen P, Andersen AB (1995) Purification and characterization of a low-molecular-mass T-cell antigen secreted by Mycobacterium tuberculosis. Infect Immun 63:1710-1717.