Anti-Influenza B virus nucleoprotein

Cat.No. HYB 116-03B

Cat. No.
HYB 116-03B
Product name
Anti-Influenza B virus nucleoprotein
Description

Mouse monoclonal antibody, biotinylated

Specificity

HYB 116-03 reacts with influenza B virus nucleoprotein as demonstrated by immunoblotting after SDS-PAGE. HYB 116-03 does not cross-react with influenza A virus.

Immunogen

Unpurified influenza B virus (B/Lee/40) for primary intranasal immunization, boosted intravenously with purified influenza B virus disrupted with Triton X-100 for 40 min at 37°C.

Application
Species reactivity (positive)
Influenza virus
Species reactivity (negative)
Not determined
Subclass

IgG2a/k

Gene ID
956539
Clone number
1B6
Epitope

Epitope differs from HYB 116-01 and HYB 116-02 as determined by inhibition ELISA.

Content

100 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Influenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The nucleoprotein of influenza B virus is a basic, phosphorylated multimeric protein consisting of 560 aa subunits that encapsidate the viral RNA genome to form a ribonucleoprotein particle.

Application 1

ELISA

Biotinylated HYB 116-03 is suitable as detecting antibody in combination with HYB 116-02 or HYB 116-01 + -02 as capture antibody in sandwich ELISA for influenza B virus (1).

1. Glikmann G, Chen S-N, Mordhorst CH, Koch C (1995) Monoclonal antibodies for the rapid diagnosis of influenza-B virus infections by ELISA: production and characterization. Clin Diagn Virol 4:27-42.