Anti-Influenza B virus nucleoprotein

Cat.No. HYB 116-02

Cat. No.
HYB 116-02
Product name
Anti-Influenza B virus nucleoprotein
Description

Mouse monoclonal antibody

Specificity

HYB 116-02 reacts with influenza B virus nucleoprotein as demonstrated by immunoblotting after SDS-PAGE. HYB 116-02 does not cross-react with influenza A virus (1).

Immunogen

Unpurified influenza B virus (B/Lee/40) for primary intranasal immunization, boosted intravenously with purified influenza B virus disrupted with Triton X-100 for 40 min at 37°C (1).

Application
Species reactivity (positive)
Influenza virus
Species reactivity (negative)
Not determined
Subclass

IgG2a/k

Gene ID
956539
Clone number
3E9
Epitope

Epitope differs from HYB 116-03 as determined by inhibition ELISA

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Influenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The nucleoprotein of influenza B virus is a basic, phosphorylated multimeric protein consisting of 560 aa subunits that encapsidate the viral RNA genome to form a ribonucleoprotein particle.

Application 1

ELISA
HYB 116-02 is suitable as capture antibody in combination with biotinylated HYB 116-03 as detecting antibody in sandwich ELISA for influenza B virus (1).

Application 2

WB
In Western blotting a dilution guideline of 1/20 has proved successful (1).

Application 3

IHC
HYB 116-02 can be used in Immunohistochemistry.
 

Application 4
IF
HYB 116-02 reacts with influenza B-infected, acetone-fixed VERO cells in immunofluorescence
cytochemistry.
 
HYB 116-02 diluted 1/1000 with a secondary polyclonal rabbit anti-mouse Ig FITC conjugated antibody (DAKO F0232) 1:20 + 1% Evans blue colors Influenza B nucleoprotein. Primarily the nucleus of the infected Vero cells is visible.
 

1. Glikmann G, Chen S-N, Mordhorst CH, Koch C (1995) Monoclonal antibodies for the rapid diagnosis of influenza-B virus infections by ELISA: production and characterization. Clin Diagn Virol 4:27-42.