Cat.No. HYB 156-01
Mouse monoclonal antibody
HYB 156-01 is specific for influenza A virus nucleoprotein and does not cross-react with influenza B virus (1).
Unpurified influenza A virus (H1N1 A/PR/8/34) for primary intranasal immunization, boosted intravenously with purified influenza A virus disrupted with Triton X-100 for 45 min at 37°C (1).
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Influenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The nucleoprotein of influenza A virus is a basic, phosphorylated multimeric protein consisting of 498 aa subunits that encapsidate the viral RNA genome to form a ribonucleoprotein particle.
HYB 156-01 is suitable as capture antibody in a sandwich ELISA for influenza A virus, using HYB 156-01B (biotinylated) for detection (1).
HYB 156-01 can be used in immunohistochemistry.
|HYB 156-01 diluted 1:1500 + secondary polyclonal goat anti-mouse Ig Fluor 555 conjugated antibody (Alexa A21424) 1:200 + Vectashield mounting medium with DAPI. DAKO IMAGEN Influenza Virus slides (S6112) were used and the entire infected Vero cells are seen.|
|HYB 156-01 diluted 1:1000 + secondary polyclonal rabbit anti-mouse Ig FITC conjugated antibody (DAKO F0232) 1:20 + 1% Evans blue colors Influenza A nucleoprotein. DAKO IMAGEN Influenza Virus slides (S6112) were used and the entire infected Vero cells are seen.|