Anti-Influenza A virus nucleoprotein

Cat.No. HYB 156-01

Cat. No.
HYB 156-01
Product name
Anti-Influenza A virus nucleoprotein
Description

Mouse monoclonal antibody

Specificity

HYB 156-01 is specific for influenza A virus nucleoprotein and does not cross-react with influenza B virus (1).

Immunogen

Unpurified influenza A virus (H1N1 A/PR/8/34) for primary intranasal immunization, boosted intravenously with purified influenza A virus disrupted with Triton X-100 for 45 min at 37°C (1).

Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG2a/k

Clone number
5D8
Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Influenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The nucleoprotein of influenza A virus is a basic, phosphorylated multimeric protein consisting of 498 aa subunits that encapsidate the viral RNA genome to form a ribonucleoprotein particle.

Application 1

ELISA
HYB 156-01 is suitable as capture antibody in a sandwich ELISA for influenza A virus, using HYB 156-01B (biotinylated) for detection (1).

Application 2

IHC
HYB 156-01 can be used in immunohistochemistry.

Application 3
IF
HYB 156-01 reacts with influenza A-infected, acetone-fixed VERO cells in immunofluorescence cytochemistry.

 
HYB 156-01 diluted 1:1500 + secondary polyclonal goat anti-mouse Ig Fluor 555 conjugated antibody (Alexa A21424) 1:200 + Vectashield mounting medium with DAPI. DAKO IMAGEN Influenza Virus slides (S6112) were used and the entire infected Vero cells are seen.
HYB 156-01 diluted 1:1000 + secondary polyclonal rabbit anti-mouse Ig FITC conjugated antibody (DAKO F0232) 1:20 + 1% Evans blue colors Influenza A nucleoprotein. DAKO IMAGEN Influenza Virus slides (S6112) were used and the entire infected Vero cells are seen.
1. Glikmann G, Mordhorst CH, Koch C (1995) Monoclonal antibodies for the direct detection of influenza-A virus by ELISA in clinical specimens from patients with respiratory infections. Clin Diagn Virol 3:361-369.