Anti-Complement factor D (human)

Cat.No. GAU 008-01

Cat. No.
GAU 008-01
Product name
Anti-Complement factor D (human)
Description

Mouse monoclonal antibody

Specificity

GAU 008-01 is specific for complement factor D in human serum and plasma (1).

Immunogen

Human factor D (1)

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
1675
Clone number
I8/1b
Epitope

Epitope specificity differs from that of GAU 010-04 (1).

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Complement factor D is a serine protease of the alternative pathway of complement activation. The protein is a single chain polypeptide with a molecular mass of 25 kDa. Factor D cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment. 

Application 1

ELISA
GAU 008-01 is used as a biotinylated detection antibody in sandwich ELISA with a GAU 010-04 capture (1). 
 

The calibration curve of a sandwich assay for Factor D using GAU 010-04 as the capture antibody and GAU 008-01B as the biotinylated detection antibody.
Application 2

WB
GAU 008-01 can be used for Western blotting following SDS-PAGE under non-reducing conditions (1, 2).

1. Oppermann M, Baumgarten H, Brandt E, Gottsleben W, Kurts C, Gotze O (1990) Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 133:181-190.

2. Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int. 40:939-947.