Anti-Complement factor D (human)

Cat.No. GAU 010-04

Cat. No.
GAU 010-04
Product name
Anti-Complement factor D (human)
Description

Mouse monoclonal antibody

Specificity

GAU 010-04 is specific for complement factor D in human serum and plasma (1).

Immunogen

Human Factor D (1)

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG2a/k

Gene ID
1675
Clone number
D10/4
Epitope

Epitope specificity differs from that of GAU 008-01 (1).

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Complement factor D is a serine protease of the alternative pathway of complement activation. The protein is a single chain polypeptide with a molecular mass of 25 kDa. Factor D cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment. 

Application 1

ELISA
GAU 010-04 can be used as a capture antibody in sandwich ELISA with biotinylated GAU 008-01 as detection antibody (1). 
 

The calibration curve of a sandwich assay for Factor D using GAU 010-04 as the capture antibody and GAU 008-01B as the biotinylated detection antibody.
Application 2

WB
GAU 010-04 can be used for Western blotting following SDS-PAGE under non-reducing conditions (2).
 

Immunoblot with GAU 010-04:

Lane 1: Purified Factor D

Lane 2: Factor D in human serum

Lane 3: SDS-PAGE purified Factor D

Lane 4: MW-marker

1. Oppermann M, Baumgarten H, Brandt E, Gottsleben W, Kurts C, Gotze O (1990) Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 133:181-190.

2. Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int. 40:939-947.