Cat.No. GAU 010-04
Mouse monoclonal antibody
GAU 010-04 is specific for complement factor D in human serum and plasma (1).
Human Factor D (1)
Epitope specificity differs from that of GAU 008-01 (1).
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Complement factor D is a serine protease of the alternative pathway of complement activation. The protein is a single chain polypeptide with a molecular mass of 25 kDa. Factor D cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment.
GAU 010-04 can be used as a capture antibody in sandwich ELISA with biotinylated GAU 008-01 as detection antibody (1).
|The calibration curve of a sandwich assay for Factor D using GAU 010-04 as the capture antibody and GAU 008-01B as the biotinylated detection antibody.|
GAU 010-04 can be used for Western blotting following SDS-PAGE under non-reducing conditions (2).
Immunoblot with GAU 010-04:
Lane 1: Purified Factor D
Lane 2: Factor D in human serum
Lane 3: SDS-PAGE purified Factor D
Lane 4: MW-marker
1. Oppermann M, Baumgarten H, Brandt E, Gottsleben W, Kurts C, Gotze O (1990) Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 133:181-190.
2. Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int. 40:939-947.