Anti-Complement component C3a/C3a(desArg)/C3 (human)

Cat.No. GAU 013-16

Cat. No.
GAU 013-16
Product name
Anti-Complement component C3a/C3a(desArg)/C3 (human)
Description

Mouse monoclonal antibody

Specificity

GAU 013-16 is specific for human C3, C3a and C3a (desArg) (1) and does not cross-react with C4a or C5a (2, 3).

Immunogen

Human C3a

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
718
Clone number
K13/16-5.7
Epitope

GAU 013-16 recognizes a different epitope on the 9 kDa C3a than GAU 017-01 (2, 3). No reaction is seen with a synthetic octapeptide representing the C3a C-terminal (2).

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Complement C3a is an anaphylatoxin of 77 amino acid residues released by the action of the C3 convertases on the N-terminal of the alpha chain of C3. It is rapidly inactivated by serum carboxypeptidase N which removes the C-terminal arginine residue generating C3a (desArg).

Application 1

ELISA
GAU 013-16 can be used as a capture antibody in sandwich ELISA with GAU 017-01 as a biotinylated detection antibody (4, 5). GAU 013-16 can also be used to immunopurify C3a (3). GAU 013-16 effectively inhibits the biological activitiy of C3a in a guinea-pig platelet activation assay (3). 
 

The calibration curve of a sandwich assay for C3a/C3a(desArg) using GAU 013-16 as the capture antibody and GAU 017-01B as the biotinylated detection antibody.

 

Application 2

WB
GAU 013-16 can be used in Western blotting (2, 3, 5).
 

Immunoblot with GAU 013-16:

Lane 1: Purified C3

Lane 2: Purifed C3 reduced/DTT

Lane 3: Purified C3a

Lane 4: EDTA-plasma

Lane 5: Zymosan-activated serum

Application 3

IHC
GAU 013-16 can be used in IHC.

IHC staining of human kidney with GAU 013-16. Strong staining of veins of nephritis kidney was seen. Dilution: 1:150.
IHC staining of human liver with GAU 013-16. Strong staining of plasma of veins in liver tissue. Dilution: 1:150.
IHC staining of human tonsil with GAU 013-16. Plasma in lumen of veins is stained strongly in tonsil. Dilution: 1:150.

 

Application 4

IF
GAU 013-16 can be used in IF.

IF staining in human kidney with GAU 013-16. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.

 

1. Oppermann M, Haubitz M, Quintin E, Götze O (1988) Complement activation in patients with renal failure as detected through the quantitation of fragments of the complement proteins C3, C5, and Factor B. Klin Wochenschr 66:857-864.

2. Nezlin R, Freywald A, Oppermann M (1993) Proteins separeted from human IgG molecules. Mol. Immunol. 30:935-940.

3. Puschel GP, Oppermann M, Muschol W, Gotze O, Jungermann K. (1989) Increase of glucose and lactate output and decrease of flow by human anaphylatoxin C3a but not C5a in perfused rat liver. FEBS Lett. 16;243(1):83-7.

4. Khodoun M, Strait R, Orekov T, Hogan S, Karasuyama H, Herbert DBR, Köhl J, Finkelman FD (2009) Peanuts can contribute to anaphylactic shock by activating complement. J Allergy Clin Immunol 123:342-351.

5. Thomas SN, van der Vlies AJ, O’Neil CP, Reddy ST, Yu SS, Giorgio TD, Swartz MA, Hubbell JA (2011) Engineering complement activation on polypropylene sulfide vaccine nanoparticles. Biomaterials 32: 2194-2203.