Anti-Complement component C3a/C3a(desArg)/C3 (human)

Cat.No. GAU 017-01

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Cat. No.
GAU 017-01
Product name
Anti-Complement component C3a/C3a(desArg)/C3 (human)
Description

Mouse monoclonal antibody

Specificity

Recognizes an epitope that is present on human C3, C3a and C3a (desArg) (1, 2). 

Does not cross-react with C4a or C5a (1, 2).

Immunogen

Human C3a (2, 3)

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
718
Clone number
D17/1
Epitope

GAU 017-01 recognizes different epitopes on the 9 kDa C3a than GAU 013-16 (1, 2). No reaction is seen with a synthetic octapeptide representing the C3a C-terminal (1).

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Complement C3a is an anaphylatoxin of 77 amino acid residues released by the action of the C3 convertases on the N-terminal of the alpha chain of C3. It is rapidly inactivated by serum carboxypeptidase N which removes the C-terminal arginine residue generating C3a (desArg).

Application 1

ELISA
GAU 017-01 can be used as a biotinylated detection antibody in sandwich ELISA with GAU 013-16 capture antibody. Does not inhibit the biological activity of C3a (1, 2, 3).

The calibration curve of a sandwich assay for C3a/C3a(desArg) using GAU 013-16 as the capture antibody and GAU 017-01B as the biotinylated detection antibody.

 

Application 2

WB
GAU 017-01 can be used in Western blotting (1, 4, 5).

1. Nezlin R, Freywald A, Oppermann M (1993) Proteins separeted from human IgG molecules. Mol. Immunol. 30:935-940.

2.  Oppermann M, Liebmann F, Götze O. (1987) Purification and quantification of human C3a anaphylatoxin using monoclonal antibodies. Complement 4:205-206

3. Oppermann M, Haubitz M, Quintin E, Götze O (1988) Complement activation in patients with renal failure as detected through the quantitation of fragments of the complement proteins C3, C5, and Factor B. Klin Wochenschr 66:857-864.

4. Puschel GP, Oppermann M, Muschol W, Gotze O, Jungermann K. (1989) Increase of glucose and lactate output and decrease of flow by human anaphylatoxin C3a but not C5a in perfused rat liver. FEBS Lett. 16;243(1):83-7.

5. Negishi A, Ono M, Handa Y, Kato H, Yamashita K, Honda K, Shitashige M, Satow R, Sakuma T, Kuwabara H, Omura K, Hirohashi S, Yamada T (2009) Large-scale quantitative clinical proteomics by labelfree liquid chromatography and mass spectrometry. Cancer Sci 100:514-519.