Anti-Thrombin (human)

Cat.No. HYB 109-03

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Cat. No.
HYB 109-03
Product name
Anti-Thrombin (human)
Description

Mouse monoclonal antibody

Specificity

HYB 109-03 binds to human thrombin and to complex of human thrombin with the thrombin inhibitor hirudin. No cross-reaction is seen with prothrombin. 

Immunogen

Thrombin isolated from activated human plasma 

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
2147
Clone number
5D10
Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Thrombin is the active enzyme that splits fibrinogen into fibrin in final stage of blood coagulation. It is generated from its circulating inactive precursor, prothrombin, by the action of factor Xa aided by factor Va, phospholipid and calcium ions. Thrombin is a glycoprotein formed by a 6-kDa A chain linked by a disulfide bridge to a 31-kDa B chain which carries the serine protease catalytic site.

Application 1

ELISA

HYB 109-03 reacts strongly in ELISA with thrombin coated directly onto the microtiter wells. Biotinylation of thrombin blocks antibody binding. 
Application 2

WB

HYB 109-03 can be used in Western blotting.

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