Anti-Thrombin (human)

Cat.No. HYB 109-04

Buy online
Cat. No.
HYB 109-04
Product name
Anti-Thrombin (human)
Description
Mouse monoclonal antibody
Specificity
HYB 109-04 binds to human thrombin and to complex of human thrombin with the thrombin inhibitor hirudin. No cross-reaction is seen with prothrombin.
Immunogen
Thrombin isolated from activated human plasma
Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass
IgG1/k
Gene ID
2147
Clone number
5G9
Epitope
Not determined
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Thrombin is the active enzyme that splits fibrinogen into fibrin in final stage of blood coagulation. It is generated from its circulating inactive precursor, prothrombin, by the action of factor Xa aided by factor Va, phospholipid and calcium ions. Thrombin is a glycoprotein formed by a 6-kDa A chain linked by a disulfide bridge to a 31-kDa B chain which carries the serine protease catalytic site
Application 1
ELISA
HYB 109-04 reacts strongly in ELISA with thrombin coated directly onto the microtiter wells. Biotinylation of thrombin blocks antibody binding. 
Application 2
WB
HYB 109-04 can be used in Western blotting (1, 2).
1. Tripathy D, Sanchez A, Yin X, Martinez J, Grammas P (2012) Age-related decrease in cerebrovascular-derived neuroprotective proteins: Effect of acetaminophen. Microvasc Res 84:278–285.
2. Yin X, Wright J, Wall T, Grammas P (2010) Brain endothelial cells synthesize neurotoxic thrombin in Alzheimer’s disease. Am J Pathol176:1600-1606.

Request

Further information