Anti-Hirudin

Cat.No. HYB 010-03

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Cat. No.
HYB 010-03
Product name
Anti-Hirudin
Description

Mouse monoclonal antibody

Specificity

The affinity (Kd) of HYB 010-03 for rHV2K47 is 4.5x10E-7 M (1). HYB 010-03 is suitable for affinity purification of hirudin forms with intact C-terminals (1).

Immunogen

Recombinant hirudin (rHV2) produced in S. cerevisiae and coupled to diphteria toxoid 

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Clone number
2D7
Epitope

The epitope of HYB 010-03 is located at the C-terminal part of hirudin. 

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Hirudin is a highly effective and very specific anticoagulant which specifically inhibits formation of fibrin and activation of factors V, VIII and XIII by forming irreversible, noncovalent 1:1 complexes with α-thrombin. Hirudin is a 7 kDa protein naturally originating from the medical leech Hirudo medicinalis.

Application 1

ELISA
HYB 010-03 can be used in ELISA  (1).

Application 2
WB
In Western blotting after SDS-PAGE, HYB 010-03 reacts with hirudin in both reduced and unreduced forms (1). 
Binding of HYB 010-03 to hirudin does not inhibit the interaction with α-thrombin,

1. Koch C, Whitechurch O, Cordier P, Roitsch C (1993) Anti-hirudin monoclonal antibodies directed toward discontinuous epitopes of the hirudin amino-terminal and epitopes involving the carboxy-terminal hirudin amino acids. Anal Biochem 214:301-312.

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