Anti-Fibrinogen (human)

Cat.No. HYB 051-04

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Cat. No.
HYB 051-04
Product name
Anti-Fibrinogen (human)
Description
Mouse monoclonal antibody
Specificity
HYB 051-04 is specific for human fibrinogen
Immunogen
Fibrinogen isolated from human plasma
Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass
IgG1/k
Gene ID
2243
Clone number
1D6
Epitope
Not determined
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Fibrinogen, the precursor of fibrin, is the coagulable protein in the blood plasma of vertebrates. Fibrinogen consists as a dimer of 3 pairs of non-identical chains a (Mr= 66,800), b (Mr= 52,000) and  g (Mr=46,500) cross-linked by disulfide bonds in their N-terminal segments. The molecule has 2 terminal D domains and one central E domain, all three domains are separated when fibrinogen is degradated by plasmin. MW is 340 kDa. Concentration in human plasma is 2-3 mg/ml.
Application 1
ELISA
HYB 051-04 reacts strongly with fibrinogen. A strong reaction is seen in ELISA with factor VIII HP directly coated onto the microtiterwell, also when tested in sandwich ELISA in combination with a polyclonal antibody against fibrinogen (eg. DAKO A 080). 
Application 2
WB
In Western blotting after SDS-PAGE, HYB 051-04 reacts with fibrinogen in non-reduced form only.

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