Anti-Factor IX (human, Christmas factor)

Cat.No. HYB 133-01

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Cat. No.
HYB 133-01
Product name
Anti-Factor IX (human, Christmas factor)
Description

Mouse monoclonal antibody

Specificity

HYB 133-01 is specific for native human factor IX.

Immunogen

Factor IX isolated from human plasma

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
2158
Clone number
1C2
Epitope

Epitope specificity differs from that of HYB 133-09 as determined by inhibition ELISA.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Coagulation factor IX is a single chain polypeptide with a molecular mass of 57 kDa. Normal concentrations of  factor IX in human plasma is app. 3 µg/ml. Factor IX deficiency and lack of coagulation activity is seen in patients suffering from the innate X-chromosome linked disorder, haemophilia B.

Application 1

ELISA

HYB 133-01 reacts strongly with native factor IX. Strong reaction is seen in ELISA with native factor IX directly coated onto the microtiter well, and also when tested in sandwich ELISA in combination with a polyclonal antibody against factor IX. 
Application 2

WB

In Western blotting after SDS-PAGE, HYB 133-01 reacts with factor IX in non-reduced form only.

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