Anti-Aprotinin

Cat.No. ABS 037-35

Cat. No.
ABS 037-35
Product name
Anti-Aprotinin
Description

ELISA, WB

Specificity

ABS 037-35 binds bovine aprotinin immobilized on a solid phase or free in solution.

Immunogen

Aprotinin from bovine lung 

Application
Species reactivity (positive)
Bovine
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
404172
Clone number
35
Epitope

The epitope of ABS 037-35 is different from the epitopes of ABS 037-18 and ABS 037-21

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Aprotinin, also called bovine pancreatic trypsin inhibitor, is a single chain globular Kunitz-type serine proteinase inhibitor found in bovine pancreas, parotid gland and lung. It forms stable, enzymatically inactive complexes with trypsin, chymotrypsin, plasmin and kallikrein. Aprotinin has been used therapeutically as an antifibrinolytic agent under the name Trasylol (Bayer) to reduce blood loss during cardiopulmonary bypass surgery but this use has been associated with an increased frequency of acute renal injury.

Application 1

ELISA

ABS 037-35 (as capture antibody coated at 1 µg/mL) forms an antibody pair with ABS 037-18 (as biotinylated detection antibody) for sandwich ELISA of aprotinin at sample concentrations of 4-250 µg/mL. Both urine and plasma samples diluted 1/1000 can be assayed against standards in buffer. PBS-based buffers are unsuitable for this assay and 0.2 M glycine sodium buffer, pH 8.8, is recommended for washing and dilution buffers.
 
The calibration curve of a sandwich assay for Aprotinin (high sensitivity) using ABS 037-21 as the capture antibody and ABS 0037-18B as the biotinylated detection antibody. 
Application 2

WB

ABS 037-35 can be used in Western blotting.