Anti-Antithrombin III (human, native)

Cat.No. HYB 230-04

Cat. No.
HYB 230-04
Product name
Anti-Antithrombin III (human, native)
Description

Mouse monoclonal antibody

Specificity

HYB 230-04 is specific for human AT III, native form.

Immunogen

Antithrombin III isolated from human plasma

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
462
Clone number
7E11
Epitope

Epitope specificity differs from that of HYB 230-01, HYB 228-10 and HYB 228-12.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Antithrombin III (AT III) is a single chain glycoprotein of molecular mass 58 kDa with four glycosylation sites and three intra-molecular disulfide bonds. AT III is normally present in human plasma at a concentration of approx. 12.5 mg/dl. It is the major inhibitor of thrombin. Inactivation of thrombin by AT-III occurs by formation of a covalent bond resulting in an inactive 1:1 stoichiometric complex between the two components. 

Application 1

ELISA

HYB 230-04 reacts strongly with native AT III. A strong reaction is seen in ELISA with native AT III coated directly onto the microtiter well, and also when used as detection antibody in sandwich ELISA in combination with a polyclonal capture antibody (eg. DAKO A 296). 
Application 2

WB

HYB 230-04 reacts with AT III in Western blotting after SDS-PAGE. 

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Further information