Anti-Antithrombin III (human)

Cat.No. HYB 228-08

Cat. No.
HYB 228-08
Product name
Anti-Antithrombin III (human)
Description

Mouse monoclonal antibody

Specificity

HYB 228-08 i specific for human AT III, native and denatured.

Immunogen

Antithrombin III isolated from human plasma 

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
462
Clone number
2 E10
Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Antithrombin III (AT III) is a single chain glycoprotein of molecular mass 58 kDa with four glycosylation sites and three intra-molecular disulfide bonds. AT III is normally present in human plasma at a concentration of approx. 12.5 mg/dl. It is the major inhibitor of thrombin. Inactivation of thrombin by AT-III occurs by formation of a covalent bond resulting in an inactive 1:1 stoichiometric complex between the two components.

Application 1

ELISA

HYB 228-08 reacts strongly with AT III both native and denatured forms. A strong reaction is seen in ELISA with both native and denatured AT III directly coated onto the microtiter well and also when used as detection antibody in sandwich ELISA in combination with a polyclonal capture antibody (eg. DAKO A 296). 
Application 2

IHC

HYB 228-08 can be used in IHC. Please consult www.proteinatlas.org
 
Paraffin-embedded human liver tissue immunostained with HYB 228-08 (www.proteinatlas.org)
Paraffin-embedded human cancerous liver tissue immunostained with HYB 228-08 (www.proteinatlas.org)

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Further information