Anti-Vitronectin (bovine, sheep)

Cat.No. CSI 004-27

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Cat. No.
CSI 004-27
Product name
Anti-Vitronectin (bovine, sheep)
Description
Mouse monoclonal antibody
Specificity
CSI 004-27 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin).
Immunogen
Lysed bovine corneal endothelial cells and extracellular matrix
Species reactivity (positive)
Bovine, sheep
Species reactivity (negative)
Human, horse
Subclass
IgG1/k
Gene ID
507525, 101119231
Clone number
A27
Epitope
Not determined
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin (1).
Application 1
ELISA
CSI 004-27 is suitable for ELISA (1, 2, 3).
Application 2
WB
CSI 004-27 is suitable for immunoblotting.
Application 3
IHC
CSI 004-27 mmunostaining of frozen PLP-fixed sections of bovine tissues. 
Application 4
AP
The antibody can be used as an affinity purification reagent of vitronectin from bovine plasma or serum and to quantitatively deplete plasma or serum of vitronectin. It can also be used to probe vitronectin conformation.
1. Underwood PA, Bennett FA (1989) A comparison of the biological activities of the cell-adhesive proteins vitronectin and fibronectin. J Cell Sci 93:641-649.
2. Underwood PA, Steele JG, Dalton BA, Bennet FA (1990). Solid phase monoclonal antibodies. A novel method of directing the function of biologically active molecules be presenting a specific concentration. J Immunol Methods 127:91-102.
3. Underwood PA, Bean PA, Mitchell SM, Whitelock JM (2001) Specific affinity depletion of cell adhesion molecules and growth factors from serum. J Immunol Methods 247:217-224.