Anti-Type III Collagen (human, dog, rat, kangaroo)

Cat.No. CSI 007-01

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Cat. No.
CSI 007-01
Product name
Anti-Type III Collagen (human, dog, rat, kangaroo)
Description
Mouse monoclonal antibody
Specificity
CSI 007-01 is highly specific for type III collagen. It has been shown to have no cross-ractivity with type I and V collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
Immunogen
Acid-digested pepsin soluble human type III collagen
Application
Species reactivity (positive)
Human, dog, rat, kangaroo
Species reactivity (negative)
Calf, sheep
Subclass
IgG1/k
Clone number
1E7-D7/Col3
Epitope
Epitope is located approximately 100nm from the N-terminal of the molecule
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Type III collagen is present in the walls of arteries, intestines and the uterus, where it strenghtens the connective tissue. Patients with defects in the type III collagen (ex. Ehlers-Danlos syndrome) are at risk of rupture of major arteries or the intestine.
Application 1
ELISA
CSI 007-01 can be used for detection of collagens by ELISA. (1,2,3,4)
Application 2
WB
In immunoblotting CSI 007-01 detects human type III collagen only in its native triple helix form (2).
Application 3
IHC
CSI 007-01 has been used successfully for immunohistology on frozen unfixed sections of human (1) and kangaroo (2) skin, and of new dog tissue associated with biomaterial implants (3, 4). If fixation of tissue is required, acetone or ethanol is recommended (1, 2, 3, 4).
Please consult www.proteinatlas.org
 
Paraffin-embedded human bronchus tissue immunostained with CSI 007-01 (www.proteinatlas.org)
1. Werkmeister JA, Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of human skin. Acta Derm Venereol 69:399-402. 
2. Stephens LJ, Werkmeister JA, Tebb TA, Ramshaw JAM (1991) Identification of type III collagen from kangaroo skin. Das Leder 42:41-44.
3. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.
4. Werkmeister JA, Glattauer V, Tebb TA, Ramshaw JAM, Edwards GA, Robets G (1991) Structural stability of long-term implants of a collagen-based vascular prosthesis. J Long Term Eff Med Implants 1:107-119