Anti-Type I Collagen (human, calf, pig, sheep, dog, goat)

Cat.No. CSI 008-01

Cat. No.
CSI 008-01
Product name
Anti-Type I Collagen (human, calf, pig, sheep, dog, goat)
Description

Mouse monoclonal antibody

Specificity

CSI 008-01 is highly specific for native type I collagen and shows no evidence of cross-reactivity with type II, III, V and VI collagens in inhibition ELISA and immunoblotting, and no evidence of cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). CSI 008-01 has a Ka of approximately 2.5 x 106 M-1 for human type I collagen.

Immunogen

Pepsin-solubilized, salt-fractionated type I collagen from human placenta (1)

Application
Species reactivity (positive)
Human, calf, pig, sheep, dog, goat
Species reactivity (negative)
Mouse, guinea pig, cat, rabbit, squirrel, horse, kangaroo, chicken
Subclass

IgG1/k

Gene ID
1281, 84032, 478835
Clone number
5D8-G9/Col1
Epitope

Epitope is located near the C-terminal of the molecule

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Type I collagen is present in connective tissue and bone where it makes up about 90% of the organic matrix. Defects in one or both alleles of the type I collagen gene causes "brittle bone disease" (osteogenesis imperfecta). Patients suffering from such defects have low levels of fully functional type I collagen resulting in fragile bones.

Application 1

ELISA
CSI 008-01 can be used to detect collagens by ELISA. 

Application 2

WB
In immunoblotting CSI 008-01 detects human type I collagen only in its native triple helix form.

Application 3

IHC
CSI 008-01 can be used for immunohistochemistry on frozen unfixed sections of human (1, 2), and bovine (3, 4) skin. If fixation of tissue is required, acetone or ethanol is recommended.(5, 6)

Sheep vagina tissue stained with CSI 008-01
Human endometrium tissue stained with CSI 008-01
Application 4

IP
CSI 008-01 can be used in Immunoprecipitation.

1. Werkmeister JA, Ramshaw JAM, Ellender G (1990) Characterisation of monoclonal antibody against native human type I collagen. Eur J Biochem 187:4369-443.

2. Werkmeister JA,  Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of human skin. Acta Derm Venereol 69:399-402.

3. Werkmeister JA, Ramshaw JAM (1988) The use of immunohistology in studies on connective tissue organisation in hides and skins. Das Leder 39:145-151. 

4. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.

5. Janssen FW, Dijkhuizen-Radersma R, Oorschot AV, Oostra J, Bruijn JD, Blitterswijk CAV (2010) Human tissue-engineered bone produced in clinically relevant amounts using a semi-automated perfusion bioreactor system: a preliminary study. J Tissue Eng Regen Med 4: 12–24.