Cat.No. CSI 008-01
Mouse monoclonal antibody
CSI 008-01 is highly specific for native type I collagen and shows no evidence of cross-reactivity with type II, III, V and VI collagens in inhibition ELISA and immunoblotting, and no evidence of cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). CSI 008-01 has a Ka of approximately 2.5 x 106 M-1 for human type I collagen.
Pepsin-solubilized, salt-fractionated type I collagen from human placenta (1)
Epitope is located near the C-terminal of the molecule
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Type I collagen is present in connective tissue and bone where it makes up about 90% of the organic matrix. Defects in one or both alleles of the type I collagen gene causes "brittle bone disease" (osteogenesis imperfecta). Patients suffering from such defects have low levels of fully functional type I collagen resulting in fragile bones.
CSI 008-01 can be used to detect collagens by ELISA.
In immunoblotting CSI 008-01 detects human type I collagen only in its native triple helix form.
CSI 008-01 can be used for immunohistochemistry on frozen unfixed sections of human (1, 2), and bovine (3, 4) skin. If fixation of tissue is required, acetone or ethanol is recommended.(5, 6)
|Sheep vagina tissue stained with CSI 008-01|
|Human endometrium tissue stained with CSI 008-01|
CSI 008-01 can be used in Immunoprecipitation.
1. Werkmeister JA, Ramshaw JAM, Ellender G (1990) Characterisation of monoclonal antibody against native human type I collagen. Eur J Biochem 187:4369-443.
2. Werkmeister JA, Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of human skin. Acta Derm Venereol 69:399-402.
3. Werkmeister JA, Ramshaw JAM (1988) The use of immunohistology in studies on connective tissue organisation in hides and skins. Das Leder 39:145-151.
4. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.
5. Janssen FW, Dijkhuizen-Radersma R, Oorschot AV, Oostra J, Bruijn JD, Blitterswijk CAV (2010) Human tissue-engineered bone produced in clinically relevant amounts using a semi-automated perfusion bioreactor system: a preliminary study. J Tissue Eng Regen Med 4: 12–24.