Cat.No. HYB 130-11
Mouse monoclonal antibody
HYB 130-11 is specific for amino acids 17-181 of human tetranectin monomer.
Tetranectin purified from human citrate plasma. Boosted before fusion with recombinant tetranectin produced in E. coli.
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Tetranectin (TN) is a serum and tissue protein, a C-type lectin, which binds to Ca++. It is a homotrimer of monomers each with a mass of 20 kDa, plasma or serum concentrations of TN are found to be approximately 10 mg/l (1). In vitro, TN can bind to kringle 4 of plasminogen and enhance the activation of plaminogen to plasmin, catalyzed by tissue plasminogen activator in the presence of poly-D-lysine. TN is best known as a prognostic marker in ovarian cancer.
HYB 130-11 was used in ELISA
In Western blotting HYB 130-11 reacts strongly with tetranectin monomer (1).
HYB 130-11 used in SDS-PAGE 12%. Tertanectin band is visable at 22kDa.
Lane1: MWM (Thermo #26621).
Lane 2: human serum (20 µL 1:50).
Lane 3 Bovine serum (20µL 1:10).
Image courtesy of Isabelle Naegelen, University of Luxemburg
Strong recognition of tetranectin in immunohistochemistry on both fresh frozen and paraffin-embedded tissues (1).
|HYB 130-11 used to stain human neutrophils A) DAPI-stained, B) tetranectin-stained, and C) overlay picture . Image courtesy of Isabelle Naegelen, University of Luxemburg|
Light microscopic immunoflorescence
HYB 130-11 can be used in light microscopic immunoflorescence (2).
1. Hogdall CK, Christiansen M, Christensen L, Yazova AK, Koch C, Clemmensen I, Norgaard-Pedersen B (1997) Monoclonal antibodies against human tetranectin, epitope characterization and use in immunohistochemistry. Clin Chim Acta 258:159-177.
2. Wernbom M, Paulsen G, Nilsen TS, Hisdal J, Raastad T (2012) Contractile function and sarcolemmal permeability after acute low-load resistance exercise with blood flow restriction. Eur J Appl Physiol 112:2051-2063.