Anti-Tetranectin (human)

Cat.No. HYB 130-11

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Cat. No.
HYB 130-11
Product name
Anti-Tetranectin (human)
Description

Mouse monoclonal antibody

Specificity

HYB 130-11 is specific for amino acids 17-181 of human tetranectin monomer.

Immunogen

Tetranectin purified from human citrate plasma. Boosted before fusion with recombinant tetranectin produced in E. coli.

Application
Species reactivity (positive)
Human, bovine
Species reactivity (negative)
Not determined
Subclass

IgG2a/k

Gene ID
7123
Clone number
5B7
Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Tetranectin (TN) is a serum and tissue protein, a C-type lectin, which binds to Ca++. It is a homotrimer of monomers each with a mass of 20 kDa, plasma or serum concentrations of TN are found to be approximately 10 mg/l (1). In vitro, TN can bind to kringle 4 of plasminogen and enhance the activation of plaminogen to plasmin, catalyzed by tissue plasminogen activator in the presence of poly-D-lysine. TN is best known as a prognostic marker in ovarian cancer.

Application 1

ELISA

HYB 130-11 was used in ELISA

Application 2

WB
In Western blotting HYB 130-11 reacts strongly with tetranectin monomer (1).
 

HYB 130-11 used in SDS-PAGE 12%. Tertanectin band is visable at 22kDa.

Lane1: MWM (Thermo #26621).

Lane 2: human serum (20 µL 1:50).

Lane 3 Bovine serum (20µL 1:10).

Image courtesy of Isabelle Naegelen, University of Luxemburg

Application 3

IHC
Strong recognition of tetranectin in immunohistochemistry on both fresh frozen and paraffin-embedded tissues (1).
 
 

Application 4

Confocal Microscopy
 

HYB 130-11 used to stain human neutrophils  A) DAPI-stained, B) tetranectin-stained, and C) overlay picture . Image courtesy of  Isabelle Naegelen, University of Luxemburg

 

Application 5

Light microscopic immunoflorescence
HYB 130-11 can be used in  light microscopic immunoflorescence (2).

1. Hogdall CK, Christiansen M, Christensen L, Yazova AK, Koch C, Clemmensen I, Norgaard-Pedersen B (1997) Monoclonal antibodies against human tetranectin, epitope characterization and use in immunohistochemistry. Clin Chim Acta 258:159-177.

2. Wernbom M, Paulsen G, Nilsen TS, Hisdal J, Raastad T (2012) Contractile function and sarcolemmal permeability after acute low-load resistance exercise with blood flow restriction. Eur J Appl Physiol 112:2051-2063.

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