Anti-Perlecan (bovine, human)

Cat.No. CSI 001-71

Cat. No.
CSI 001-71
Product name
Anti-Perlecan (bovine, human)
Description

Mouse monoclonal antibody

Specificity

CSI 001-71 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).

Immunogen

Bovine corneal endothelial cells

Species reactivity (positive)
Bovine, human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
444872, 3339
Clone number
A71
Epitope

Epitope is located in domain I

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling.

Application 1

ELISA

CSI 001-71 can be used in ELISA
Application 2

WB

In Western blotting a dilution guideline of 1/100 has proved successful (1).
Application 3

IHC

CSI 001-71 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.
Application 4

AP

CSI 001-71 can be used in affinity chromatography to separate recombinant domain I from tissue culture fluid (1).
Application 5

IP

CSI 001-71 can be used in immunoprecipitation.

1. Whitelock JM, Murdoch AD, Iozzo RV, Underwood PA (1996) The degradation of human endothelial cell-derived perlecan, and release of bound bFGF by stromelysin, plasmin and heparanases. J Biol Chem 271:10079-10086.

 
2. Muthusamy, A, Cooper, CR, Gomes, RR JR. (2010) Soluble perlecan domain i enhances vacular endothelial growth factor-165 actibity and receptor phosphorylation in human bone marrow endothelial cells, BMC Biochem 11:43. doi: 10.1186/1471-2091-11-43.