Anti-Perlecan (bovine, human)

Cat.No. CSI 001-76

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Cat. No.
CSI 001-76
Product name
Anti-Perlecan (bovine, human)
Description

Mouse monoclonal antibody

Specificity

CSI 001-76 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).

Immunogen

Lysed bovine corneal endothelial cells and extracellular matrix

Species reactivity (positive)
Bovine, human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
444872, 3339
Clone number
A76
Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling (1).

Application 1

ELISA

CSI 001-76 can be used in ELISA.
Application 2

WB

CSI 001-76 can be used in Western blotting (1).
Application 3

IHC

CSI 001-76 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.
Application 4

AP

CSI 001-76 is more sensitive than CSI 001-71 or CSI 001-74 in ELISA (2).
Application 5

IP

CSI 001-76 can be used in immunoprecipitation

1. Whitelock JM, Graham LD, Melrose J, Murdoch AD, Iozzo R, Underwood PA (1999) Human perlecan immunopurified from different endothelial cell sources has different adhesive properties for vascular cells. Matrix Biol 18:163-178.

2. Whitelock JM, Murdoch AD, Iozzo RV, Underwood PA (1996) The degradation of human endothelial cell-derived perlecan, and release of bound bFGF by stromelysin, plasmin and heparanases. J Biol Chem 271:10079-10086.