Anti-Fibronectin (bovine, human)

Cat.No. CSI 005-32

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Cat. No.
CSI 005-32
Product name
Anti-Fibronectin (bovine, human)
Description

Mouse monoclonal antibody

Specificity

CSI 005-32 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). The antibody inhibits cell adhesion to fibronectin mediated by the heparin-binding domain. 

Immunogen

Bovine corneal endothelial cells

Species reactivity (positive)
Bovine, human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
280794 (2335)
Clone number
A32
Epitope

Epitope is located in the 40kD Hep II heparin-binding domain, but differs from that of CSI 005-35

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities (1).

Application 1

ELISA

CSI 005-32 can be used in ELISA. It can be used to probe fibronectin conformation and to quantitate plasma fibronectin in a sandwich ELISA with antibody CSI 005-35. (1, 2, 3, 4)
Application 2

WB

In Western blotting dilution guideline of 1/100 has proved successful (1).
Application 3
IHC
CSI 005-32 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues. 
Application 4

IP

CSI 005-32 can be used in immunoprecipitation.

1. Underwood PA, Dalton BA, Steele JG, Bennett FA, Strike P (1992) Anti-fibronectin antibodies that modify heparin binding and cell adhesion: evidence for a new cell binding site in the heparin binding region. J Cell Sci 102:833-845.

2. Underwood PA,  Steele JG, Dalton BA (1993) Effects of polystyrene surface chemistry on biological activity of solid phase fibronectin and vitronectin, analysed with monoclonal antibodies. J Cell Sci 104:793-803.

3. Di Girolamo N, Underwood PA, McCluskey PJ, Wakefield D (1993) Functional activity of plasma fibronectin in patients with Diabetes mellitis. Diabetes 42:1606-1613.

4. Dalton BA, McFarland CD, Underwood PA, Steele JG (1995) Role of heparin binding domain of fibronectin in attachment and spreading of human bone derived cells. J Cell Sci 108:2083-2092.