Anti-Fibronectin (bovine)

Cat.No. CSI 005-22

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Cat. No.
CSI 005-22
Product name
Anti-Fibronectin (bovine)
Description

Mouse monoclonal antibody

Specificity

CSI 005-22 is highly specific for bovine fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin).

Immunogen

Bovine corneal endothelial cells on beads

Species reactivity (positive)
Bovine
Species reactivity (negative)
Human
Subclass

IgG1/k

Gene ID
280794
Clone number
A22
Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities (1).

Application 1

ELISA

CSI 005-22 can be used in ELISA (1,2,3)
Application 2

WB

CSI 005-22 can be used in immunoblotting
Application 3

IHC

CSI 005-22 can be used i in immunostaining of frozen PLP-fixed sections of bovine tissues.
Application 4

IP

CSI 005-22 can be used in immunoprecipitation

1. Underwood PA, Dalton BA, Steele JG, Bennett FA, Strike P (1992) Anti-fibronectin antibodies that modify heparin binding and cell adhesion: evidence for a new cell binding site in the heparin binding region. J Cell Sci 102:833-845.

2. Underwood PA,  Steele JG, Dalton BA (1993) Effects of polystyrene surface chemistry on biological activity of solid phase fibronectin and vitronectin, analysed with monoclonal antibodies. J Cell Sci 104:793-803.

3. Underwood PA, Steele JG, Dalton BA, Bennett FA (1990) Solid phase monoclonal antibodies. A novel method of directing the function of biologically active molecules by presenting a specific orientation. J Immunol Methods 127:91-102.