Anti-yENaC (y−subunit epithelial sodium channel, neoepitope)

Cat.No. CAM 035-01

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Cat. No.
CAM 035-01
Product name
Anti-yENaC (y−subunit epithelial sodium channel, neoepitope)
Description

Mouse monoclonal antibody

Specificity

CAM 035-01 is specific for the neoepitope generated after proteolytic cleavage of human yENaC and recognizes the C-terminal fragment.

Immunogen

Synthetic peptide seguence: VGGSIIHKAC

Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Clone number
11-35-1
Epitope

CAM 035-01 is specific for the neoepitope generated after proteolytic cleavage of human ENaC and recognizes the C-terminal fragment starting from amino acid residue 182.

Content

Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the y-subunit. In the y-subunit, putative cleavage sites for prostasin, kallikrein (similar site as prostasin), elastase, and plasmin exist. Prostasin is a glycosylphosphatidylinositol anchored apical serine protease expressed in the collecting duct that activates yENaC at a site close to but in the C-terminal direction to the furin cleavage with putative release of a 43-residue inhibitory tract.

Application 1

ELISA
CAM 035-01 can be used in ELISA.

Application 2

WB
CAM 035-01 can be used in Western blot.

Application 3

IHC
CAM 035-01 can be used in IHC.

Immunohistochemical staining of a formalin-fixed, paraffin-embedded kidney tissue with CAM 035-01. Courtesy of Per Svenningsen, PhD.
Application 4

IF
CAM 035-01 can be used in immunofluorescence.

1. Zachar RM, Skjødt K, Marcussen N, Walter S, Toft A, Nielsen MR, Jensen BL, Svenningsen P. The Epithelial Sodium Channel g-Subunit Is Processed Proteolytically in Human Kidney. J Am Soc Nephrol. 2014 Jul 24. pii: ASN.2013111173. (epub ahead of print).

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