Cat.No. CAM 035-01
Mouse monoclonal antibody
CAM 035-01 is specific for the neoepitope generated after proteolytic cleavage of human yENaC and recognizes the C-terminal fragment.
Synthetic peptide seguence: VGGSIIHKAC
CAM 035-01 is specific for the neoepitope generated after proteolytic cleavage of human ENaC and recognizes the C-terminal fragment starting from amino acid residue 182.
Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the y-subunit. In the y-subunit, putative cleavage sites for prostasin, kallikrein (similar site as prostasin), elastase, and plasmin exist. Prostasin is a glycosylphosphatidylinositol anchored apical serine protease expressed in the collecting duct that activates yENaC at a site close to but in the C-terminal direction to the furin cleavage with putative release of a 43-residue inhibitory tract.
CAM 035-01 can be used in ELISA.
CAM 035-01 can be used in Western blot.
CAM 035-01 can be used in IHC.
|Immunohistochemical staining of a formalin-fixed, paraffin-embedded kidney tissue with CAM 035-01. Courtesy of Per Svenningsen, PhD.|
CAM 035-01 can be used in immunofluorescence.
1. Zachar RM, Skjødt K, Marcussen N, Walter S, Toft A, Nielsen MR, Jensen BL, Svenningsen P. The Epithelial Sodium Channel g-Subunit Is Processed Proteolytically in Human Kidney. J Am Soc Nephrol. 2014 Jul 24. pii: ASN.2013111173. (epub ahead of print).