Anti-yENaC (y−subunit epithelial sodium channel)

Cat.No. CAM 003-07

Buy online
Cat. No.
CAM 003-07
Product name
Anti-yENaC (y−subunit epithelial sodium channel)
Description

Mouse monoclonal antibody

Specificity

CAM 003-07 is specific for the inhibitory tract of human yENaC subunit.

Immunogen

The inhibitory peptide from the human yENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human yENaC subunit.

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Clone number
3c7
Epitope

The epitope is on the inhibitory tract of human yENaC. The epitope differs from that of CAM 005-02.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the t-subunit and putative release of a 43-amino acid inhibitory tract from the y-subunit ectodomain.

Application 1

ELISA
CAM 003-07 was used in ELISA. A sandwich ELISA can be made using CAM 003-07 (1-4 μg/ml) as the capture antibody and biotinylated CAM 005-02 (0.05 - 0.2 μg/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131).

 
The calibration curve of a sandwich assay for Anti-yENaC using CAM 003-07 as the capture antibody and CAM 005-02B as the biotinylated detection antibody. 
Application 2

WB
CAM 003-07 was used in Western Blot.

 
WB performed with CAM 003-07 on a lysate of HEK293 cells transfected with and without γENaC.

Lane 1 loaded with 5µg

Lane 2 loaded with 10µg

Lane 3 loaded with 20 µg

Application 3

IHC
CAM 003-07 was used in immunohistochemistry.

1. Svenningsen P, Uhrenholt TR, Palarasah Y, Skjodt K, Jensen BL, Skott O (2009) Prostasin-dependent activation of epithelial Na+ channels by low plasmin concentrations. Am J Physiol Regul Integr Comp Physiol 297:R1733-R1741.

2. Zachar RM, Skjødt K, Marcussen N, Walter S, Toft A, Nielsen MR, Jensen BL, Svenningsen P. The Epithelial Sodium Channel g-Subunit Is Processed Proteolytically in Human Kidney. J Am Soc Nephrol. 2014 Jul 24. pii: ASN.2013111173. (epub ahead of print).