Cat.No. CAM 003-07
Mouse monoclonal antibody
CAM 003-07 is specific for the inhibitory tract of human yENaC subunit.
The inhibitory peptide from the human yENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human yENaC subunit.
The epitope is on the inhibitory tract of human yENaC. The epitope differs from that of CAM 005-02.
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the t-subunit and putative release of a 43-amino acid inhibitory tract from the y-subunit ectodomain.
CAM 003-07 was used in ELISA. A sandwich ELISA can be made using CAM 003-07 (1-4 μg/ml) as the capture antibody and biotinylated CAM 005-02 (0.05 - 0.2 μg/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131).
|The calibration curve of a sandwich assay for Anti-yENaC using CAM 003-07 as the capture antibody and CAM 005-02B as the biotinylated detection antibody.|
CAM 003-07 was used in Western Blot.
|WB performed with CAM 003-07 on a lysate of HEK293 cells transfected with and without γENaC. |
Lane 1 loaded with 5µg
Lane 2 loaded with 10µg
Lane 3 loaded with 20 µg
CAM 003-07 was used in immunohistochemistry.
1. Svenningsen P, Uhrenholt TR, Palarasah Y, Skjodt K, Jensen BL, Skott O (2009) Prostasin-dependent activation of epithelial Na+ channels by low plasmin concentrations. Am J Physiol Regul Integr Comp Physiol 297:R1733-R1741.
2. Zachar RM, Skjødt K, Marcussen N, Walter S, Toft A, Nielsen MR, Jensen BL, Svenningsen P. The Epithelial Sodium Channel g-Subunit Is Processed Proteolytically in Human Kidney. J Am Soc Nephrol. 2014 Jul 24. pii: ASN.2013111173. (epub ahead of print).