Anti-Factor XII (human, heavy chain)

Cat.No. CAM 011-37

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Cat. No.
CAM 011-37
Product name
Anti-Factor XII (human, heavy chain)
Description

Mouse monoclonal antibody

Specificity

CAM 011-37 binds the heavy chain of human factor XII, including factor XII derivatives such as α-factor XIIa and α-factor XIIa C1-inhibitor complexes.

Immunogen

Affinity purified human factor XII.

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Clone number
10-11-37
Epitope

Differs from CAM 011-05.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Factor XII is a component in the coagulation cascade. It is a plasma glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII to a-factor XIIa, which in turn activates factor XI to factor XIa.

Application 1

ELISA
CAM 011-37 was used in ELISA. CAM 011-37 as a capture antibody (1.6 μg/mL) was used in combination with biotinylated CAM 011-05 (1μg/mL) to measure total factor XII, including factor XII derivatives such as α-factor XIIa and α-factor XIIa C1-inhibitor complexes (1).

 
The calibration curve of a sandwich assay for Anti-Factor XII using CAM 011-37 as the capture antibody and CAM 011-05B as the biotinylated detection antibody. 
Application 2

WB
CAM 011-37 was used in western blot to detect factor XII, α-factor XIIa C1-inh complexes and α-factor XIIa (1).

1. Madsen DE, Sidelmann J, Overgaard K, Koch C, Brodersen Gram J (2013) ELISA for determination of total coagulation factor XII concentration in human plasma. J Imm Meth 394:32–9.