Anti-Butyrylcholinesterase (human, BChE)

Cat.No. HAH 002-01B

Cat. No.
HAH 002-01B
Product name
Anti-Butyrylcholinesterase (human, BChE)

Mouse monoclonal antibody, biotinylated


HAH 002-01 is specific for butyrylcholinesterase from human serum or plasma.


Butyrylcholinesterase isolated from human plasma

Species reactivity (positive)
Species reactivity (negative)
Not determined


Gene ID
Clone number

Not determined


50 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.

0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
4-8ºC without exposure to light. No precautions necessary during handling.

Butyrylcholinesterase (BChE, EC is synthetizised in the liver, and is predominantly found in serum, liver and pancreas. Butyrylcholinesterase is a tetrameric glycoprotein (molecular mass of 350 kDa), and consists of four subunits, each with molecular mass of 90 kDa.

Application 1
HAH 002-01 reacts with BChE in normal human serum in sandwich ELISA, using HAH 002-01 as both capture and detection antibody (1). Serum cholinesterase activity can be measured by enzyme antigen immunoassay (EAIA) in combination with HAH 002-01 as capture antibody (2, 3). HAH 002-01 is also applicable in sophisticated immunomagnetic quantification assays for the detection of nerve agent adducts (4, 5).
The calibration curve of a sandwich assay for Butyrylcholinesterase using HAH 002-01 as the capture antibody and HAH 002-01B as the biotinylated detection antibody.

1. Aoki Y, Helzlsouer K, Strickland P (2014) Arylesterase Phenotype-Specific Positive Association Between Arylesterase Activity and Cholinesterase Specific Activity in Human Serum. Int. J. Environ. Res. Public Health 11:1422-1443.

2. Pan Y, Gao D, Yang W, Cho H, Yang G, Tai HH, Zhan CG (2005) Computational redesign of human butyrylcholinesterase for anticocaine medication. Proc Natl Acad Sci 102:16656-61.

3. Yang W, Pan Y, Zheng F, Cho H, Tai HH, Zhan CG (2009) Free-Energy Perturbation Simulation on Transition States and Redesign of Butyrylcholinesterase. Biophysical Journal 96:1931-1938.

4. Sporty J, Lemire S, Jakubowski E, Renner J, Evans R, Williams R, Schmidt J, van der Schans M, Noort D, Johnson R (2010) Immunomagnetic Separation and Quantification of Butyrylcholinesterase Nerve Agent Adducts in Human Serum. Anal Chem 82, 6593-6600.

5. Knaack J, Zhou Y, Abney C, Prezioso S, Magnuson M, Evans R, Jakubowski E, Hardy K, Johnson R (2012) High-Throughput Immunomagnetic Scavenging Technique for Quantitative Analysis of Live VX Nerve Agent in Water, Hamburger, and Soil Matrixes. Anal Chem 84:10052-10057.