Anti-α-fetoprotein (human, AFP)

Cat.No. HYB 097-04

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Cat. No.
HYB 097-04
Product name
Anti-α-fetoprotein (human, AFP)
Description

Mouse monoclonal antibody

Specificity

HYB 097-04 is specific for human AFP. 

Immunogen

Human α-fetoprotein isolated from amniotic fluid 

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
174
Clone number
10B8
Epitope

Epitope belongs to group II

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
α-fetoprotein (AFP) is a serum protein produced by hepatocytes. AFP levels are used as a tumor marker for hepatomas and germ cell tumors, and the serum concentration has prognostic significance in other diseases. Levels of AFP is 0.6-9.3 kIU/L in blood from normal individuals under the age of 40. Concentrations raise with age and during pregnancy.
AFP consists of one single polypeptide chain of 590 aminoacids with MW 65 kDa.
Application 1

ELISA
Ka=190, determined as concentration of antibody giving half-maximal binding to hepatoma-AFP bound to solid phase (unit: 108 L/mol). HYB 097-04 reacts strongly with human AFP. A strong reaction is seen when tested in sandwich ELISA in combination with a polyclonal antibody against human AFP (eg. DAKO A0008).

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